Table 2.
Antibiotic resistance genes identified in CI133 and CI172 strains
| A-CARD softwad results | CI133 strain | CI172 strain | ||||||||
| Gene | Product | Resistance | Hit Type | Ident (%) | Score | E-value | Contig* | Score | E-value | Contig* |
| cat | Chloramphenicol acetyltransferase | ARO:3,000,387: phenicol antibiotic | Perfect | 100 | 465,692 | 4,60E-162 | NODE_154 | 465,692 | 5,00E-165 | NODE_208 |
| hns | Global nucleic acid-binding transcriptional dual regulator H-NS | ARO:3,000,008: penam | Perfect | 100 | 276,944 | 2,56E-88 | NODE_17 | 276,944 | 3,00E-92 | NODE_462 |
| evgA | DNA-binding response regulator in two-component regulatory system EvgAS | ARO:3,000,662: norfloxacin | Perfect | 100 | 417,157 | 1,93E-142 | NODE_106 | 417,157 | 2,00E-145 | NODE_158 |
| tetD | Transposon Tn10 TetD protein | ARO:0,000,051: tetracycline | Perfect | 100 | 283,493 | 7,89E-92 | NODE_624 | 283,493 | 8,00E-95 | NODE_307 |
| sat | Streptothricin acetyltransferase | ARO:3,000,034: nucleoside antibiotic | Perfect | 100 | 365,925 | 2,88E-123 | NODE_170 | 365,925 | 3,00E-126 | NODE_6 |
| marA | DNA-binding transcriptional dual activator of multiple antibiotic resistance | ARO:0,000,001: fluoroquinolone antibiotic | Perfect | 100 | 266,544 | 1,63E-84 | NODE_122 | ND | ––– | ––– |
| B- ResFinder: Identifcation of acquired antibiotic | CI133 strain | CI172 strain | ||||||||
| Gene | Phenotype | Accession No | Identity | Contig | Position in contig | Identity | Contig | Position in contig | ||
| blaOXA-1 | Amoxicillin, amoxicillin + clavulanic acid, ampicillin, ampicillin + clavulanic acid, cefepime, piperacillin, piperacillin + tazobactam | HQ170510 | 100 | NODE_154 | 7038..7868 | 100 | NODE_208 | 113..943 | ||
| catA1 | Chloramphenicol | V00622 | 100 | NODE_154 | 303..962 | ND | –– | –– | ||
| aadA1 | Spectinomycin, streptomycin | JQ480156 | 100 | NODE_170 | 1950..2738 | 100 | NODE_6 | 3482..4270 | ||
| dfrA1 | Trimethoprim | X00926 | 100 | NODE_170 | 3415..3888 | 100 | NODE_6 | 2332..2805 | ||
| mdf(A) | Unknown macrolide, aminoglycoside, tetracycline, fluoroquinolone phenicol, and rifamycin | Y08743 | 100 | NODE_175 | 32,621..33847 | ND | –– | –– | ||
| sitABCD | Hydrogen peroxide | AY598030 | 100 | NODE_224 | 596..4045 | ND | –– | –– | ||
| tetB | Doxycycline, tetracycline, minocycline | AF326777 | 100 | NODE_624 | 1153..2358 | 100 | NODE_307 | 1151..2356 | ||
ND Not determined, Contig* The contig containing drugs determinant resistance gene was identified by BlastN