Figure 1.

Intravital microscopy reveals the presence of single tumor cells at the invasive front of BCBM. (A) Schematic representation of the generation of a BCBM model derived from PyMT, E-cad-mCFP tumor. (B) Representative image (n = 4 mice) obtained by multi-photon imaging through a cranial window of a BCBM invasive front, 2 weeks after tumor implantation. The image depicts tumor cells expressing H2B-Dendra2 (green) grown in ROSA26^mTmG mice. The mouse brain parenchyma expressed tdTomato (mTmG; grey). The invasion front shows the presence of single tumor cells expressing H2B-Dendra2 (green) that are disconnected from the tumor lobules and associated with the brain vasculature (mTmG, grey). (C) Representative pictures obtained by multi-photon imaging through a cranial window of BCBM 2 weeks after tumor implantation. Images show single tumor cells expressing H2B-Dendra2 (green) in close association with blood vessels. Blood vessels were imaged with 70 kDa fluorescent-dextran (grey).