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. 2022 Jun 28;14(13):3171. doi: 10.3390/cancers14133171

Figure 2.

Figure 2

Anti-ADAM10 mAb 8C7 preferentially binds human colon tumour cells compared to normal cells. (A) Single cell isolates from colon tumour and matched normal patient tissue were stained with the anti-ADAM10 antibodies 8C7 and 4A11, and with antibodies against CD45 and EpCam, and analysed by flow cytometry. Histograms show normalised staining of epithelial cell populations (EpCam positive, CD45 negative). (B) Tumours were recovered from mice bearing two human colorectal PDX lines, and single cell isolates were stained with 8C7, 4A11 or control IgG, before analysis by flow cytometry. Normal colon and spleen were also analysed for 8C7 binding (human-specific 4A11 was not used). Histograms show overlaid binding of ADAM10 antibodies with an isotype control. (C) Quantitation of 8C7 binding to tumour cell isolates, from multiple passages of six distinct colorectal PDX tumour lines (n = 14 tumours), compared to normal tissue (% positive cells). Binding of the anti-human ADAM10 mAb 4A11 to PDX tumour cells is also shown for comparison.