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. 2022 Jun 28;14(13):3164. doi: 10.3390/cancers14133164

Figure 4.

Figure 4

TCR stimulation upregulates GPR56 expression on TILs and enhances the activation status of T cells. (A) ES-2scFvCD3 and T cells (isolated from healthy human PBMC donors or from TILs, from digested ovarian cancer tumor samples) (Effector: Target ratio 10:1) co-cultured for 4 days. GPR56 expression analyzed within the CD3, CD4 and CD8 fractions at day 0 and day 3 of the co-culture. (B) ES-2scFvCD3 or FaDuscFvCD3 co-cultured with Jurkat WT, Jurkat GPR56 and Jurkat empty vector for 24 h. Expression levels of CD69 and CD25 were analyzed on the different Jurkat cell lines after 24 h of co-culture. 2way ANOVA’s were performed to determine significance. (C) CD81 expression on CD3-positive Jurkat GPR56 cells. (D) Luminescence from Jurkat NFAT Luc and Jurkat NFAT Luc GPR56 upon a 24-h co-culture with ES2-anti-CD3 at a 6:1 ratio or Jurkat cells alone (n = 4). Across the figure, t-test values: * = p < 0.05, ** = p < 0.01, **** = p < 0.0001, all error bars in the figure represent SD.