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Selection and identification of a CRISPR/Cas9-edited TOP2α E19/I19 5′ SS clonal cell line. (A) qPCR results from K/VP.5 and K/VP.5/edit-3 cells using wild-type and edited specific E19/I19 boundary qPCR probes. (B) Genomic sequencing of the TOP2α E19/I19 boundary in K/VP.5 and K/P.5/edit-3 cells. Republished with permission of the American Society for Pharmacology and Experimental Therapeutics, from Hernandez et al. Mol Pharmacol. 99:226–241, 2021 [23].
