. 2022 Jul 4;12(13):1729. doi: 10.3390/ani12131729

Table 2.

Sequences used for real-time PCR primers.

Genes	Primers (5′→3′)	Product Size	Gene Bank ¹
Occludin	Sense: GTTACTACTACAGCCCCTTGTTGG	142 bp	NM_205128.1
Occludin	Antisense: AGCAGGATGACGATGAGGAA	142 bp	NM_205128.1
Claudin-1	Sense: AAGAAGATGCGGATGGCTGT	158 bp	NM_001013611.2
Claudin-1	Antisense: AAGAGGGCTGATCCAAACTCAA	158 bp	NM_001013611.2
ZO-1	Sense: CTTCAGGTGTTTCTCTTCCTCCTC	131 bp	XM_015278980.2
ZO-1	Antisense: CTGTGGTTTCATGGCTGGATC	131 bp	XM_015278980.2
TLR4	Sense: TTCGGTTGGTGGACCTGAATCTTG	114 bp	NM_001030693.1
TLR4	Antisense:ACAGCTTCTCAGCAGGCAATTCC	114 bp	NM_001030693.1
FAK	Sense: CTGTCCTACGCCGACCTCAT	74 bp	NM_205435.1
FAK	Antisense: TTGCTGTCACCCTTATCCTTG	74 bp	NM_205435.1
MyD88	Sense: AAGGTGTCGGAGGATGGTGGTC	120 bp	NM_001030962.4
	Antisense: GGAATCAGCCGCTTGAGACGAG	120 bp	NM_001030962.4
IRAK4	Sense: TGGTTCGCTGCTTGACAGACTTG	98 bp	NM_001030738.1
	Antisense: TGATGCCATTCGCAGTACCTTGAG	98 bp	NM_001030738.1
β-actin	Sense: ATTGTCCACCGCAAATGCTTC	113 bp	NM_205518.1
	Antisense:AAATAAAGCCATGCCAATCTCGTC

ZO-1, Zonula occudens-1; TLR4, Toll-like receptor 4; FAK, focal adhesion kinase; MyD88, myeloid differentiation factor 88; IRAK4, IL-1R-associated kinase 4. ¹ Genbank Accession Number.