Table 5.

Effects of dietary Gln supplementation on mRNA expression of tight junction adhension of intestine of broilers challenged with LPS ¹.

Item	Saline		LPS		SEM	p value
	Ala	Gln	Ala	Gln		LPS	Gln	LPS × Gln
Duodenum
Claudin-1	1.05	1.50	0.85	1.11	0.069	0.001	0.006	0.323
Occludin	1.03	1.18	0.77	0.85	0.042	<0.001	0.019	0.383
ZO-1	1.04	1.18	0.71	0.92	0.051	0.006	0.115	0.936
Jejunum
Claudin-1	1.00	1.47	0.41	0.89	0.088	<0.001	<0.001	0.840
Occludin	1.01	1.25	0.73	0.91	0.056	0.001	0.013	0.699
ZO-1	0.99	1.26	0.65	0.87	0.062	<0.001	0.005	0.748
Ileum
Claudin-1	1.01	1.37	0.63	0.89	0.066	<0.001	<0.001	0.398
Occludin	1.07	1.21	0.72	0.84	0.066	0.004	0.234	0.879
ZO-1	1.00	1.24	0.87	0.99	0.044	0.011	0.016	0.382

Ala, alanine; Gln, glutamine; ZO-1, Zonula occludens protein 1. Values are means ± SEM, n = 10 (10 chicken per treatment); ¹ Broilers were treated with LPS or saline injection.