Table 6.

Effects of dietary Gln supplementation on mRNA expression of TLR4/FAK/MyD88 signaling in intestine of broilers challenged with LPS ¹.

Item	Saline		LPS		SEM	p Value
	Ala	Gln	Ala	Gln		LPS	Gln	LPS × Gln
Duodenum
TLR4	1.00 b	0.74 c	1.46 a	0.97 b	0.042	<0.001	<0.001	0.003
FAK	1.06	0.62	1.21	0.81	0.051	0.037	<0.001	0.785
MyD88	1.03	0.77	1.26	1.03	0.058	0.031	0.029	0.852
IRAK4	1.08	0.67	1.26	1.05	0.051	0.001	<0.001	0.230
Jejunum
TLR4	1.04	0.56	1.31	0.98	0.066	0.003	0.001	0.479
FAK	1.03	0.71	1.21	0.90	0.044	0.013	<0.001	0.988
MyD88	1.01	0.53	1.35	0.87	0.064	0.002	<0.001	0.998
IRAK4	1.01	0.77	1.26	0.91	0.057	0.038	0.004	0.541
Ileum
TLR4	1.01	0.68	1.63	1.12	0.059	<0.001	<0.001	0.186
FAK	1.01	0.88	1.14	0.96	0.025	0.016	0.0011	0.548
MyD88	1.05 a	0.48 c	1.11 a	0.84 b	0.042	<0.001	<0.001	0.001
IRAK4	1.04	0.57	1.27	0.88	0.072	0.038	0.002	0.749

Ala, alanine; Gln, glutamine; TLR4, Toll-like receptor 4; FAK, focal adhesion kinase; MyD88, myeloid differentiation factor 88; IRAK4, IL-1R-associated kinase 4. Values are means ± SEM n = 10 (10 chicken per treatment). Means in a row with superscripts without a common letter differ, p < 0.05; ¹ Broilers were treated with LPS or saline injection.