FIGURE 2:

Integrin preparations and metal ion effects on α5β1^WT. (A) SDS–PAGE (7.5% polyacrylamide) of α5β1^WT and α5β1^{D137A, D138A} under nonreducing and reducing conditions. The α5 chain is divided into two disulfide-linked fragments by furin cleavage. (B) FP assay showing the effect of metal ions on the binding of α5β1^WT (20 nM) to FITC-cRGD peptide (5 nM) in pH 7.4 Tris-buffered saline. Titrations were done in triplicate.