FIGURE 1:

Representative nuclear shapes and EFC ratio differences in control vs. TP53- or MTA2-siRNA transfected MCF10A cells. (A) Shapes of lamin A/C stained nuclei with corresponding EFC ratios overlaid on each nucleus in a representative image of control (nontarget siRNA transfected, left), TP53-siRNA transfected (middle) and MTA2-siRNA transfected (right) MCF10A epithelial cells at 120 h post-siRNA treatment without drug treatment (vehicle-control 0.5% DMSO only). Scale bars represent 50 µm. (B) Mean EFC ratios differences (+ SEM) for the three siRNA treatment groups without compound treatment (with vehicle control 0.5% DMSO only). Between-group analysis was done using two-tailed Mann–Whitney U test in GraphPad. P values were <0.0001 (depicted as **** in the graph); the statistical comparison included 32601 nuclei for nontarget siRNA, 25362 nuclei for TP53 siRNA, and 6191 nuclei for MTA2 siRNA.