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. 2022 May 12;33(6):tp1. doi: 10.1091/mbc.E21-10-0506

FIGURE 2:

FIGURE 2:

Multi-instrument microscopy experiments examine FA dynamics and architecture in hPSCs. (A) Representative SD confocal image depicting paxillin-positive FAs located at the cell edge (green) and cell interior (magenta). Selected time points of 0 min (green), 50 min (cyan), and 100 min (magenta) are shown for both edge and center FAs. White regions indicate highly stable FAs. (B) Corresponding images of the inset square region shown in A. (C–F). Lateral (top) and axial (bottom) projections of iPALM images of (C) Eos-tagged integrin β5, (D) paxillin, (E) Eos-tagged actin, and (F) Eos-tagged α-actinin. Color scale in each bottom panel represents axial position as noted. Scale bar = 1 µm. (G) Average (and standard deviation) axial positions of hPSC cornerstone FA components as determined from iPALM images. Images are reproduced with permission from Stubb, Guzmán, et al. (2019).