Figure 6.

Adaptation to standardized cell culture conditions described by standard operating procedures (SOPs) under a Quality Management System (QMS) results in improved genomic stability of cultured hPSCs. Survival analysis (Kaplan–Meier curves) of the evolution of the probability (%) of hPSCs being affected by de novo genomic alterations over time (passages in vitro) before and after adaptation to standardized cell culture conditions, using G-banding karyotyping (A) and Comparative Genome Hybridization array (aCGH; B) as detection methods. For each panel, the top, middle and bottom plots show the probability of hPSCs acquiring polymorphic alterations, pathogenic alterations and total alterations, respectively. Pre-adaptation (PrA) and post-adaptation (PoA) refer to hPSCs analyzed before and after completing adaptation to the standard cell culture conditions used in our laboratory, respectively. Error bars represent standard error (S.E.M.). FPA: Frequent Polymorphic Alteration; CNV: Copy Number Variation; can: Copy Number Alteration.