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. 2022 Jun 24;11(13):2017. doi: 10.3390/cells11132017

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of H2AX phosphorylation (serine 139) (phospho-H2AX) in HT29/ALDH1B1 and HT29/mock cells. (a) Forty (40) μg of HT29/ALDH1B1 and HT29/mock cell extracts were subjected to western blot analysis against H2AX phosphorylation. Histone H3 was used as a control for equal loading. (b) The protein expression levels of the phosphorylated (ser139) H2AX were >1.5-fold and >1.8-fold higher under normal and etoposide conditions, respectively, compared to HT29/mock cells. * p < 0.05, ** p < 0.01.