. 2022 Jun 28;11(13):2047. doi: 10.3390/cells11132047

Table 1.

List of solvents used for phosphopeptide enrichment in the four methods.

Method	Loading Buffer	Washing Buffer 1	Washing Buffer 2	Washing Buffer 3	Elution Buffer 1	Elution Buffer 2
Glutamic acid	65% ACN, 2% TFA, saturated glutamic acid	65% ACN, 0.5% TFA	65% ACN, 0.1% TFA	____	4% NH₃·H₂O	4% NH₃·H₂O, 50% ACN
Lactic acid	70% ACN, 5% TFA, 20% Lactic acid	30% ACN, 0.5% TFA	80% ACN, 0.4% TFA	____	4% NH₃·H₂O	4% NH₃·H₂O, 50% ACN
Glycolic acid	80% ACN, 5% TFA, 1 M Glycolic acid	80% ACN, 1% TFA	20% ACN, 0.1% TFA	____	4% NH₃·H₂O	4% NH₃·H₂O, 50% ACN
DHB	80% ACN, 5% TFA, 20 mg/ml DHB	30% ACN, 1% TFA	50% ACN, 1% TFA	80% ACN, 1% TFA	4% NH₃·H₂O	4% NH₃·H₂O, 50% ACN