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. 2022 Jun 29;11(13):2064. doi: 10.3390/cells11132064

Figure 6.

Figure 6

TXNRD1 protein levels in mutant TP53-expressing cells correlates with GPX abundance and activity. (A) Relative abundance of TXNRD1 protein levels in TP53 null H1299 cells (H1299), or H1299 cells expressing tetracycline inducible plasmids containing either wild-type TP53 (WTP53) or one of the designated TP53 mutation types was measured by quantitative LC MS/MS following 48 h treatment with 10 µM tetracycline. (B) TXNRD1 protein abundance is inversely correlated to GPX protein abundance in H1299 TP53 null, WTP53, and TP53 mutant-expressing cell lines. (C) TXNRD1 mRNA expression was measured by qPCR in human cancer cell lines expressing endogenous WT TP53, or one of the TP53 mutation types indicated. (D) GPX activity in cells expressing endogenous WT and mutant TP53 was measured indirectly via a coupled reaction with glutathione reductase and normalized to total protein. * Denotes statistical difference from respective control, * p < 0.05. Superscripts (a, b, c) denote statistical difference between TP53 subtypes. Subtypes with the same superscripts are not statistically different. Error bars indicated SEM.