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. 2022 Jun 30;11(13):2093. doi: 10.3390/cells11132093

Figure 3.

Figure 3

The expressions of ECM and hair growth markers upon minoxidil treatment in the 3D and 2D systems. (A) The gene expressions of the ECM markers VCAN and NCAM after 48 h. (B) The gene expressions of the hair growth markers CTNNB1, ALPL, and FGF1 after 48 h. For A and B, the mRNA level was quantified using TaqMan real-time PCR. The results represent the mean ± SD of three independent experiments. (C) β-catenin, ALP, and FGF1 protein expressions after 48 h. The band intensities were assessed using LICOR. The results represent the mean ± SD of three independent experiments. * p ≤ 0.05, ** p ≤ 0.01.