Table 2.
Critical levels of germination and corresponding 5mC levels calculated according to a p.dose function constructed on GLM models on combined data from all seeds, and separate data from non-desiccated seeds at MC 12.5–14.5% and desiccated seeds at MC 7.6–7.8%.
| Assumed Germination Level | Predicted DNA Methylation Level (%) 1 | ||
|---|---|---|---|
| All Seeds | Non-Desiccated Seeds | Desiccated Seeds | |
| 95% | 9.375 ± 0.382 | 8.767 ± 0.435 | 9.859 ± 0.48 |
| 90% | 8.347 ± 0.237 | 8.018 ± 0.28 | 8.742 ± 0.369 |
| 80% * | 7.989 ± 0.181 * | 7.638 ± 0.216 * | 8.208 ± 0.274 * |
| 50% | 7.221 ± 0.147 | 7.111 ± 0.161 | 7.294 ± 0.265 |
| 25% | 6.613 ± 0.198 | 6.657 ± 0.197 | 6.57 ± 0.394 |
| 10% | 6.005 ± 0.283 | 6.204 ± 0.278 | 5.845 ± 0.565 |
| 5% | 5.591 ± 0.347 | 5.895 ± 0.344 | 5.353 ± 0.69 |
* Viability critical for storage at gene banks. 1 Data significant at the level of p < 0.0001.