. 2022 Jun 29;11(13):2067. doi: 10.3390/cells11132067

Table 1.

Important Motifs for Active AAA-ATPases are Conserved only in Pex1/Pex6 D2 ATPase Domains.

		Walker A	Pore Loop 1	Walker B	ISS	Arg Finger
		ATP Binding	Substrate Binding	ATP Hydrolysis	Inter-Subunit Signaling	Assembly, ATP Hydrolysis
Consensus		`GxxGxGKT`	`+Ω`Φ`x-`	ΦΦΦΦ`DE`	`DGF`	`ALLRPGR`
	ScPex1	`GKQGIGKT`	`CETLHE-TSNLDKTQ`	`LIVLDN`	`QVTKI`	`LLFDKHF`
	HsPEX1	`GGKGSGKS`	`CKALR--GKRLENIQ`	`VVLLDD`	`MIKEF`	`LLV…VHI`
	AtPEX1	`GPPGSGKT`	`CSTLA--LEKVQHIH`	`VIILDD`	`VIDDY`	`TLSSSGR`
D1	ScPex6	`S…NNVGKA`	`CLSLTSNSRQLDSTS`	`VIFLAH`	`LLDDF`	`SFRS--H`
	HsPEX6	`GPPGCGKT`	`CSSLC--AESSGAVE`	`VLLLTA`	`LLLNE`	`DVQ--TA`
	AtPEX6	`GIPGCGKR`	`CHSLL--ASSERKTS`	`ILLLRH`	`VIREL`	`TIR--RC`
	ScPex1	`GYPGCGKT`	`GPEIL--NKFIGASE`	`ILFFDE`	`QMDGA`	`ALLRPGR`
	HsPEX1	`GPPGTGKT`	`GPELL--SKYIGASE`	`ILFFDE`	`QLDGV`	`ALLRPGR`
D2	AtPEX1	`GPPGCGKT`	`GPELL--NKYIGASE`	`ILFFDE`	`ELDGV`	`ALLRPGR`
	ScPex6	`GPPGTGKT`	`GPELL--NMYIGESE`	`VIFFDE`	`ELDGM`	`ALLRPGR`
	HsPEX6	`GPPGTGKT`	`SPELI--NMYVGQSE`	`IIFFDE`	`ELDGL`	`ALLRPGR`
	AtPEX6	`GPPGTGKT`	`GPELI--NMYIGESE`	`VIFFDE`	`EMDGM`	`ALLRPGR`

Structural alignments were generated with MUSTANG [155] based on AlphaFold2 structures. Grey residues match consensus sequences for the given motif [149,150] Sc: Saccharomyces cerevisiae, Hs: Homo sapiens, At: Arabidopsis thaliana.