FIG. 3.

SDS-PAGE and renaturing SDS-PAGE analysis of the purified six-His-tagged L. citreum Mur protein. Coomassie blue staining was used for lanes 1 and 2. Lane 1, whole SDS cell extract of E. coli XL1-Blue harboring pTIL343 induced for 4 h with isopropyl-β-d-thiogalactopyranoside; lane 2, recombinant L. citreum Mur purified on Ni-nitrilotriacetic acid resin; lane 3, activity of the purified recombinant protein by renaturing SDS-PAGE containing 0.2% autoclaved M. lysodeikticus cells. The molecular masses (in kilodaltons) of standard proteins are indicated on the left.