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Renaturing SDS-PAGE analysis of the chimeric Mur-cA protein expressed in L. lactis MG1363acmAΔ1. Lane 1, L. lactis MG1363acmAΔ1 harboring plasmid pTIL344; lane 2, L. lactis MG1363acmAΔ1 harboring pTIL344 and pNZ9520 carrying nisRK genes. SDS cell extract of each strain was prepared from a noninduced culture and loaded onto polyacrylamide gel containing 0.2% autoclaved M. lysodeikticus cells. The molecular masses (in kilodaltons) of standard proteins are indicated on the left of the gel.
