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. 2001 Feb;67(2):858–864. doi: 10.1128/AEM.67.2.858-864.2001

TABLE 2.

Oligonucleotides used in this study

Primera Sequenceb Restriction site Positionsc
LnP1 GTNATGATNGCNCARGC Conserved region; aa 86-91d
LnP2(r) GGRTCNGTNGCRTANC Conserved region; aa 190-195d
LnP3(r) CATTCCAACTCGTGCCATGA nt 683 to 664 of the sequenced fragment
LnP4 CGGCCCAATATCAGCATGTT nt 684 to 703 of the sequenced fragment
LnP5 CGCGGATCCACTTTTTGGTCGCAAACAC BamHI nt 305 to 323 of the sequenced fragment
LnP6(r) GGAAGCTTCGGAACATTTCCGTT HindIII nt 857 to 843 of the sequenced fragment
LnP7 GCCTGCAGCAAAAAGAAAAAGACGT PstI nt 216 to 232 of the sequenced fragment
LnP8(r) CCATCGATTTTTTGTGGTACATCAAAGCG ClaI nt 838 to 818 of the sequenced fragment
LnP9 CCATCGATGCTGGTACTTCTAATTCCGGT ClaI nt 652 to 675 of acmAe
LnP10(r) CCGCTCGAGTCATCTTCAGTCTGTTTTAAAAG XhoI nt 1382 to 1359 of the 3′ end of the transcription terminator downstream acmAe
a

(r), complementary primer. 

b

N = A or C or T or G; R = A or G. Restriction sites are underlined. 

c

aa, amino acid; nt, nucleotide. 

d

Positions refer to the L. lactis MG1363 AcmA sequence (9). 

e

Positions refer to the L. lactis IL1403 acmA sequence (37).