Expression of cmg1 during sclerotial parasitism. RNA was extracted from dual cultures of C. minitans and S. sclerotiorum (P) and from mycelium of S. sclerotiorum (S) or C. minitans (C) grown alone, as controls. Five micrograms of RNA was loaded onto a formaldehyde gel and hybridized to the radiolabelled cmg1 gene. The cultures were 3, 5, and 10 days old (3d, 5d, and 10d, respectively) when samples were removed. The bottom panel shows ethidium bromide-stained rRNA as an indication of the amounts of RNA loaded. Representative results of three independent experiments are shown.