Figure 3.

Relative mRNA expression of the genes involved in NLRP3 inflammasome. Lung fibroblast cell line (MLG) was used as reference point for all examined genes expression. In order to analyze relative expression, comparative ΔCT method was employed. This method is a convenient way to calculate relative gene expression levels between different samples in that it directly uses the threshold cycles generated by the qRT-PCR system for calculation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.