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PFKM downregulation promoted cell apoptosis and inhibited OXPHOS and glycolysis. H9c2 cells were transfected with PFKM siRNA or siNC, and (A) cell viability, (B, C) TUNEL staining, (D, E) expression of PFKM, Bcl-2 and Bax, (F) OCR, (G) ECAR, (H) ATP and (I) lactate level were measured. Scale bar: 100 μm. ***P < 0.001 vs siNC. (see Supplementary Fig. S9–S12, more information for Supplementary Fig. S12 can be found in Supplementary Information 1.)
