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. 2022 Jul 8;12:11684. doi: 10.1038/s41598-022-15743-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PFKM regulates DOX-mediated cell growth and apoptosis via the glycolysis pathway. PFKM-overexpressing H9c2 cells were treated with DOX with/without 2-DG for 24 h, and (A) cell viability and (B, C) TUNEL staining was measured. Scale bar: 100 μm. ***P < 0.001 vs Control; ^##P < 0.01, ^###P < 0.001 vs DOX + vector + vehicle; ^ΔΔP < 0.01, ^ΔΔΔP < 0.001 vs DOX + PFKM + vehicle.