Figure 1.

Class-I PI3K is needed for Salmonella survival in B cells. B cells were purified from the spleens of C57BL/6 mice and infected at a MOI of 50 with Salmonella. Subsequently, the cells were lysed and plated on LB Agar at the indicated times. (A) CFUs were recovered from infected B cells and treated 1 h before and during infection with the indicated concentrations of wortmannin or (B) treated with the PI3K class I inhibitor AZD8186 (500 nM). (C) B cells were infected at a MOI of 50 with Salmonella WT, Salmonella ∆sopb, Salmonella ∆sopb pC460S (phosphatase-inactive domain), or Salmonella ∆sopb psopb (WT SopB). Percentage of Salmonella survival in (A–C) was calculated by dividing CFUs recovered at 24 h between CFUs recovered at 1 h. Results are expressed as means ± SDs. n = 3. A two-way ANOVA test was used for multiple comparisons, and an unpaired Student’s t-test was used for bars. * p < 0.05, ** p < 0.01, and *** p < 0.001.