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. 2022 Jul 2;23(13):7392. doi: 10.3390/ijms23137392

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of 5-demethyl NOB on cell apoptosis and differentiation. THP-1 cells were treated with vehicle or 5-demethyl NOB (20 and 40 μM) for 48 h, and cell apoptosis was detected by flow cytometric analysis. (a) A representative histogram of cell apoptosis. (b) The percentage of apoptotic cells was quantified. (c–e) Western blot analysis of apoptosis-related gene expression after 5-demethyl NOB treatment for 48 h. (f) THP-1 cells were treated with vehicle or 5-demethyl NOB (20 and 40 μM) for 24 h and 48 h. CD11b mRNA expression was measured by RT–qPCR analysis. The data represent the mean ± SD of three independent experiments. ** p < 0.01 represents significant differences compared to the vehicle-treated group.

Effects of 5-demethyl NOB on cell apoptosis and differentiation. THP-1 cells were treated with vehicle or 5-demethyl NOB (20 and 40 μM) for 48 h, and cell apoptosis was detected by flow cytometric analysis. (a) A representative histogram of cell apoptosis. (b) The percentage of apoptotic cells was quantified. (c–e) Western blot analysis of apoptosis-related gene expression after 5-demethyl NOB treatment for 48 h. (f) THP-1 cells were treated with vehicle or 5-demethyl NOB (20 and 40 μM) for 24 h and 48 h. CD11b mRNA expression was measured by RT–qPCR analysis. The data represent the mean ± SD of three independent experiments. ** p < 0.01 represents significant differences compared to the vehicle-treated group.