Figure 3.

Enrichment of INADL and MUC1 in ductal tissues. Parotid and submandibular frozen tissue sections were separated visually by laser capture microscopy (LCM) into acinar/myoepithelial and ductal fractions and prepared for quantitative polymerase chain reaction (qPCR) assessment of transcript expression (A, B). Note that submandibular tissue architecture was preserved, whereas parotid tissue structure was compromised by freezing (bottom panels visualized by U6 in situ hybridization), leading to more “compartment mixing” during laser capture (C). INADL, MUC1, and AQ5 transcripts were examined for 1 parotid patient sample and 2 submandibular patient samples. INADL and MUC1 were enriched in the ductal isolation, particularly for the submandibular samples. AQ5 marked the acinar population. INADL was not detected in the “clean” acinar isolation from the submandibular gland but was detected by qPCR in dissected ducts in both submandibular patient samples.