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. 2022 Feb 26;101(8):931–941. doi: 10.1177/00220345221075215

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Figure 4. — Loss of Gnas transformed chondrocytes by activating Hedgehog (Hh). (A) Schematics of mice mating. (B) Whole-mount X-gal staining of Prrx1-Cre; Gnas^fl/fl; Ptch1^LacZ mouse and control littermate calvarias. The arrow (red) indicates ectopic X-gal (green) in outlined sutures and fontanels. Scale bar: 0.5 mm. (C) X-gal staining on coronal cryostat sections of Prrx1-Cre; Gnas^fl/fl; Ptch1^LacZ mouse calvaria. The arrow (red) indicates X-gal⁺ chondrocytes (green) in the outlined cartilage. Scale bar: 100 μm. (D) Quantification of X-gal staining. Percentage of X-gal⁺ chondrocytes among total chondrocytes. N = 6 mice. Mean ± SD. Two-tailed t test. (E) Costaining with Safranin O and von Kossa on X-gal staining of Prrx1-Cre; Gnas^fl/fl; Ptch1^LacZ mouse and control littermate calvarias. The arrow (green) indicates X-gal⁺ chondrocytes contributing to the mineralization of mutant cartilage (outlined). Scale bar: 100 μm.