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. 2022 Jun 28;23(13):7185. doi: 10.3390/ijms23137185

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Study design—The effect of predator scent-stress (PSS) on corticosterone levels and behavioral responses. After habituation period of seven days, animals were exposure to PSS or sham-PSS for 15 min. Urine samples were collected 1–2 h after PSS exposure for corticosterone levels. Behaviors were assessed on day 7 using the elevated plus maze (EPM) and the acoustic startle response (ASR) assay. On day 8, rats were sacrificed, and their brains were collected for immunoreactivity analyses of Iba-1 and CRXCR1 in the hippocampus subregions.