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. 2022 Jun 28;23(13):7185. doi: 10.3390/ijms23137185

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Study design—Effects of high-dose hydrocortisone, RU486 (GC receptor antagonist-mifepristone) or saline immediately post-exposure on behavioral stress responses at day 7 post-exposure. After habituation period of seven days, animals were exposure to PSS or sham-PSS for 15 min. Animals were exposed to predator scent stress (PSS), treated for 1 day with hydrocortisone 25 mg/kg, saline or RU486 30 mg/kg and assessed behaviorally with the elevated plus maze (EPM) and acoustic startle response (ASR) tests at day 7. On day 8, rats were sacrificed, and their brains were collected for immunoreactivity analyses of Iba-1 in the hippocampus subregions.