Figure 2.

Average percentage of total reads mapping to host in each depletion treatment. All treatments significantly reduced host reads relative to untreated controls (t-test, p < 0.01). Error bars are ±SE. Untreated A and custom A are comparing 20 oral and 5 cloacal swabs. Untreated B and custom B include 14 cloacal swabs and 28 tissue samples. MAMribo (NEBNext rRNA depletion kit, human/mouse/rat; New England Biolabs) and CXribo (riboPOOL kit; siTOOLS Biotech) were tested on all 20 oral and 19 cloacal swabs (1 CXribo-treated sample did not have sufficient read depth for comparison). See also data presented in Table 1.