Figure 4.

Relationship between Newcastle disease virus (NDV) L-test gene RT-qPCR Ct value and viral read yield under different RNA depletion treatments. Shown are oral and/or cloacal samples from Tanzania for which there were viral reads present and measurable Ct values (n = 14). Ct of 26–27 was the threshold for obtaining significant reads, and custom depletion yielded the greatest number of viral reads at a given Ct value. Dotted lines are lines of best fit for data points. MAMribo = NEBNext rRNA depletion kit, human/mouse/rat, New England Biolabs; Cxribo = riboPOOL kit, siTOOLS Biotech.