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. 2022 Jun 28;23(13):7159. doi: 10.3390/ijms23137159

Figure 1.

Figure 1

ERβ is highly expressed in OCSCs. (A–C), ES2, OV90, and OVSAHO cells were subjected to ALDEFLUOR assay and the expression of ERβ isoforms 1, 2, 4, and 5 in ALDH-ve and ALDH+ve cells was determined using RT-qPCR. (D–F), SKOV3, ES2, and A2780 cells were cultured as adherent (2D) and spheroid (3D) cultures and the expression of ERβ isoforms 1, 2, 4, and 5 was determined using RT-qPCR. (G,H), ALDH-ve and ALDH+ve cells enriched from SKOV3 and A2780 cells were seeded on fibronectin coated plates and after 24 h cells were fixed in 4% paraformaldehyde and subjected to ICC staining with ERβ antibody as described in methods. (I,J), ALDH-ve and ALDH+ve populations isolated from SKOV3 and A2780 cells were treated with vehicle or indicated concentrations of LY500307 for 96 h, and the cell viability rates were determined using Cell-Titer-Glo assay. Data are represented as mean ± SE. ns: no significance; * p < 0.05; *** p < 0.001; **** p < 0.0001.