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. 2022 Jun 21;23(13):6884. doi: 10.3390/ijms23136884

Figure 3.

Figure 3

Orcinol stain and anti-Gb3Cer, anti-Gb4Cer, Stx1a and Stx2a TLC overlay assays of the neutral GSL preparations of pHRPTEpiCs (A) and pHCoEpiCs (B). Applied GSL amounts of pHRPTEpiCs (A) correspond to 5 × 106 cells (orcinol stain), 2 × 106 cells using the anti-Gb3Cer and anti-Gb4Cer antibody, respectively, and 6 × 105 cells for the Stx1a and Stx2a TLC overlay assay, respectively. Applied GSL amounts of pHCoEpiCs (B) are equivalent to 5 × 106 cells (orcinol stain), 5 × 105 cells using an anti-Gb3Cer and anti-Gb4Cer antibody, respectively, and 1 × 106 cells for the Stx1a and Stx2 TLC overlay assay, respectively. MHCs, monohexosylceramides. For further details refer to Detzner and collaborators [193,194], where the data were taken from.