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. 2022 Jun 21;23(13):6884. doi: 10.3390/ijms23136884

Figure 5.

Figure 5

Distribution of Gb3Cer, Gb4Cer, and cholesterol to sucrose gradient fractions F1 to F8 prepared from pHRPTEpiCs (A) and pHCoEpiCs (B). Anti-Gb3Cer and anti-Gb4Cer antibodies were employed for immunostaining and manganese(II)chloride for cholesterol (Chol) detection. Standard (S) equivalents of 2 µg and 0.2 µg of reference neutral GSLs from human erythrocytes and 1 µg of reference cholesterol (Chol) were applied as positive controls for the anti-Gb3Cer and anti-Gb4Cer TLC overlay assay and the cholesterol detection, respectively. DRMs, detergent-resistant membranes. For further details refer to Detzner and collaborators [193,194], where the data were taken from.