Table 1.
Summary of plant metabolomics studies of different model species using various high-throughput metabolomics technologies *.
| Plant | Stress | Method | Tissue | Type of Metabolites | Refs |
|---|---|---|---|---|---|
| A. thaliana | Heat shock | GC-MS | Root and shoot | Glyceric acid, maltose, asparagine, glutamine, glycine, and trehalose | [91] |
| LC-MS/MS | Leaf | Phenylalanine-derived metabolites | [92] | ||
| Drought | LC/UPLC-MS/MS; GC-MS |
Hormones, MGDG, DGDG, SQDG, PC, PE, PS, PI, and TAG | [93] | ||
| Heat primed, heat shock | UPLC-MS/MS; HILIC/UPLC-MS/MS |
Amino acid, carbohydrate, lipid, nucleotide, 2-isopropyl malate, dihydrokaempferol, putrescine, 2-hydroxy laurate, glycerol 3-phosphate, glutathione, ascorbate, tocopherol, and GPC | [94] | ||
| High light, cold | GC-TOF MS; LC-MS/MS |
Citrate, gluconic acid, hexose, amino acid, organic acid, sugar | [95] | ||
| GC-TOF-MS | Sucrose phosphate, starch, serine, raffinose, pyruvate, malate, and proline | [96] | |||
| GC-TOF-MS | Sucrose, glucose, and fructose | [97] | |||
| Bacteria | UPLC-MS/MS | Hormone and redox metabolites | [98] | ||
|
Genetic
modification of AtTSPO |
1H-NMR; HPTLC |
Seed and leaf | Fatty acids, lipids, sterols, esters, TAG, FFA, DAG, and starch | [99] | |
| Drought, heat | FT-ICR-MS; GC-TOF-MS |
Leaf | Sucrose, sorbitol, coniferyl alcohol, cinnamyl alcohol, and fatty acids | [100] | |
| Brachypodium distachyon | Drought | GC–MS | Leaf | Fatty acid, malic acid, amino acids | [101] |
| Lotus japonicus | Agrobacterium | LC-IonTrap-MS/MS; NMR |
Flower buds and petals | Flavonoids, quercetagetin, gossypetin | [102] |
| Physcomitrella patens | Salt, cold, abscisic acid |
GC-MS | Protonema tissue | Sugar, amino acid, and organic acid | [103] |
| High night temperatures |
GC-TOF-MS | Leaf | Amino acids, sugars, organic acids, phenylpropanoids, phosphates, and polyhydroxy acids | [104] | |
| Oryza sativa | Drought | GC-MS; LC | Leaf | Citric and aconitic acids, benzoic acid, carbohydrates, proline, norvaline, GABA, benzoic acid, TCA cycle acids, and sugars | [105] |
| Salinity | GC-TQMS | Leaf and root | Mannitol, sugar, and organic acid | [106] | |
| Hordeum vulgare | Salinity | LC-MS/MS; MALDI–MSI | Root | PC, fatty acyls, glycerophospholipid, glycerolipid, prenol lipid, polyketide, sphingolipid, DAG, TAG, and SQDAG |
[107] |
| GC-MS; LC-MS/MS |
Root and leaf | Phytohormones and chlorophyll | [108] | ||
| Drought, bacteria | LC-MS/MS | Leaf | Flavonoid, auxin, flavonol, flavanone, anthocyanin, and hormones |
[109] | |
| Medicago sativa; M. truncatula | Drought | LC-TQMS | Leaf and root | Flavonoid, carbohydrate, abscisic acid, and proline | [110] |
| M. truncatula | Drought, Fusarium oxysporium |
LC-MS/MS | Organic acid, sugar, citrate, isocitrate, and tetrahydroxychalcone | [111] | |
| Histone deacetylase inhibitor | LC-MS/MS; GC-MS | Seed and seedling |
Amino acid, lipid, and carbohydrate; saccharopine, UDP-glucose/UDP-galactose, 1-linolenoylglycerol, 1-linoleoyl-GPI 182, creatine, and N-acetylglutamine | [112] |
* The detailed list of metabolomics studies in other plants is provided in Supplementary Table S1. GC-MS: Gas chromatography-mass spectrometry; LC-MS/MS: Liquid chromatography-tandem mass spectrometry; UPLC: Ultra performance LC; HILIC: Hydrophilic interaction chromatography; HPTLC: High-performance thin-layer chromatography; TQMS: Triple quadrupole MS; 1HNMR: proton nuclear magnetic resonance; FT-ICR-MS: fourier-transform ion cyclotron resonance MS; MALDI-MSI: matrix-assisted laser desorption ionization MS imaging; IonTrap-MS/MS: ion trap MS/MS; GC-TOF-MS: GC-time of flight MS; MGDG: Monogalactosyldiacylglycerol; DGDG: Digalactosyldiacylglycerol; SQDG: Sulfoquinovosyldiacylglycerol; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PS: phosphatidylserine; PI: phosphatidylinositol; TAG: Triacylglycerides; GPC: Glycerophosphorylcholine; SQDAG: Sulfoquinovosyl diacylglycerol; FFA: Free fatty acids; DAG: Diacylglycerol; GABA: Gamma aminobutyric acid; AtTSPO: A. thaliana translocator protein.; UDP: Uridine diphosphate.