FIG. 2.

Nucleic acids isolated, fractionated, labeled with lissamine-rhodamine B, and fragmented on a silica syringe-operated column. (A) Isolated total nucleic acids (lane 1), partially fractionated DNA (lane 2), purified DNA (lane 3), and purified RNA (lane 4) from B. subtilis were analyzed by electrophoresis in 1% agarose. M, λ-HindIII DNA marker. (B) Total nucleic acids from B. thuringiensis fractionated in a denaturing 7.5% polyacrylamide gel (46) before (lane 1) and after (lanes 2 and 3) labeling and fragmentation; fluorescence of labeled and fragmented product before (lane 3) and after (lane 2) ethidium bromide staining. M, single-stranded 20- and 50-base size markers.