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. 2022 Jun 28;23(13):7200. doi: 10.3390/ijms23137200

Figure 1.

Figure 1

Characterization of EVs derived from plasma of patients with brain tumors. Plasma EVs were purified using ExoEasy and ExoQuick kits. (A) Nanoparticle Tracking Analysis showing examples of typical size and concentrations of the plasma EVs of GBM (a) and MMA (b) by ExoEasy. (B) Transmission electron microscope image showing ExoEasy purified GBM EVs, which are 100–200 nm diameters in size. (C) Western blots run on Native conditions demonstrated the presence of EV marker CD63 in GBM and HC plasma EVs. EVs were purified from plasma with ExoEasy (XOE) and ExoQuick (XOQ) followed by electrophoresis in Native gel. The blot was detected with mouse anti-human CD63 (1:1000). The CD63 proteins showed a much large size at 250 KD due to heavy glycosylation under native conditions, rather than the 25 KD under denaturing conditions. We found that CD63 protein was more abundant in Exo-Quick-purified EVs than in Exo-Easy-purified EVs.