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. 2022 Jun 24;23(13):7050. doi: 10.3390/ijms23137050

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The characteristics of hepatocytes (HepG2 cells) after the activation by media control (control) and the preparations from K. pneumoniae (Kleb) or C. albicans (Can) alone or the combination (Kleb+Can) as indicated by supernatant cytokines (IL-6, TNF-α, and IL-10) at 72 h post-incubation (A–C) and the gene expression (4 h post-incubation) of the molecules of inflammation (IL-6, TNF-α, and IL-10) (D–F) and apoptosis (casp8, casp9, and bcl-2) (G–I). (Independent triplicated experiments were performed.) #, p < 0.05; ##, p < 0.01; ###, p < 0.001; ####, p < 0.0001 vs. control; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, the indicated groups as have determined by ANOVA with Tukey’s analysis.