Figure 2.

Fructose enhances RAGE expression through GLUT, and dapagliflozin reverses NADPH oxidase subunit (p67−phox) in the fructose-induced type 2 DM lens. (A,B) Representative images showing GLUT5− and RAGE-positive (green) and p47−phox and NOX4−positive (red) cells in the lens with or without dapagliflozin. Quantitative data were compared to no fructose group. Cell nuclei were counterstained in DAPI (blue). (C,D) RAGE, SGLT2, GLUT1, GLUT5, p67-phox and NOX1−4 protein expressions in the fructose-induced type 2 DM lens were significantly decreased by dapagliflozin. Values are presented as mean ± SEM (n = 6 for each group). Scale bar = 20 μm. * p < 0.05 and ** p < 0.001; ^# p < 0.05 versus Fructose 8 W.