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. 2022 Jun 29;23(13):7252. doi: 10.3390/ijms23137252

Figure 2.

Figure 2

Hemichannels formed by Cys mutants do not open upon exposure to divalent cation free solution (DCFS). HeLa cells transfected with wild-type Cx46 or Cys mutants were placed in recording media containing 10 μM DAPI under control conditions or in DCFS. Pictures of 16 cells were taken every 20 s for 20 min, with 4 replicates. DAPI uptake was calculated as arbitrary units per second (AU/Sec). (A) Control conditions. The rate of DAPI uptake was similar in cells expressing all Cys mutants, except for the C186A mutant, which showed a small, but significant higher, DAPI uptake compared to cells expressing wild-type Cx46 (*, p < 0.05). (B) DCFS. HeLa cells expressing wild-type Cx46 showed an enhanced rate of DAPI uptake, which was not observed in any of the Cys mutants (***, p < 0.001) (n = 4 for each condition).