Table 1.

Site-directed mutagenesis primers for hCx46 extracellular Cys mutations.

Primer Name	5′ to 3′ Sequence
Cx46-C54A Forward	cagtcagacttcaccgccaacacccagcagcc
Cx46-C54A Reverse	ggctgctgggtgttggcggtgaagtctgactg
Cx46-C61A Forward	ccagcagccgggcgccgagaacgtctgc
Cx46-C61A Reverse	gcagacgttctcggcgcccggctgctgg
Cx46-C65A Forward	gctgcgagaacgtcgcctacgacagggcct
Cx46-C65A Reverse	aggccctgtcgtaggcgacgttctcgcagc
Cx46-C181A Forward	ccgctctaccgcgccgaccgctggcc
Cx46-C181A Reverse	ggccagcggtcggcgcggtagagcgg
Cx46-C186A Forward	cgaccgctggcccgcccccaacacggtg
Cx46-C186A Reverse	caccgtgttgggggcgggccagcggtcg
Cx46-C192A Forward	cccaacacggtggacgccttcatctccaggcc
Cx46-C192A Reverse	ggcctggagatgaaggcgtccaccgtgttggg