FIG. 5.

(a) Agarose gel electrophoresis of plasmids isolated from DPC220 (lanes 1 and 10), DPC721 (lanes 2 and 11), and seven BIMs of DPC220 origin (lanes 3 to 9) generated by challenge with a cocktail of phages. The positions of plasmids pAH82, pAH33, and pAH90 are indicated. (b) Gel obtained by PCR using primers designed to one of the hybrid hsdS determinants (orf17) of the cointegrate plasmid pAH90. A PCR product of approximately 1.03 kb in length is only possible when a cointegrate form is present. Lanes are the same as in panel a.