Figure 2.

Dipotassium glycyrrhizinate (DPG) inhibits cell migration and cancer stem-like cells in a melanoma cell line. (A) SK-MEL-28 cells treated with DPG fill the wound area (the area between the two dotted lines) more slowly at 24, 48, and 72 h compared to untreated cells (p-value < 0.001). Graphics also shows the average and standard deviation of three independent experiments. (B) Loss of sphere-forming ability of SK-MEL-28 cells after DPG-exposure for 24 h (p-value = 0.008). At 48 h, DPG-treated cells were not available for analysis. The third image on the right shows just the enlargement of the images on the left. The graphic shows the average and standard deviation of three independent experiments. (C) DPG significantly increases miR-4443 (p-value = 0.02) and miR-3620 (p-value = 0.01) expression in SK-MEL-28 cell line. (D) DPG decreases cluster of differentiation 209 (CD209) and Tenascin (TNC) mRNA levels in SK-MEL-28 (p-value = 0.018 and p-value = 2.38 × 10⁻⁶, respectively). For all assays, data represent means and standard deviations of a representative experiment performed in triplicate. Statistics were performed in a two-tailed T-test with an alpha error of 0.05.