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MS analysis of rpTcCathL1 and rTcCathL1. Peptides identified by peptide fingerprinting using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) are marked in red; peptides sequenced by MS/MS analysis are underlined; amino acid residues of the active center are marked in green. The proposed start of the mature enzyme is indicated by a black arrow. (A) Hydrolysis by glutamate-specific peptidase, (B) hydrolysis by trypsin.
