Figure 3.

PDAC Patients Have Altered Circulating Immune Populations. Peripheral blood mononuclear cells (PBMCs) were collected and processed from non-cancer and PDAC patients, stained with isotope-bound antibodies, and analyzed by the mass cytometer. Biological replicates represent staining and analyses of n = 8 non-cancer and n = 13 PDAC patient samples. (A) Representative gating for granulocytes, T cells, B cells, monocytes, dendritic cells, and natural killer cells; and (B) Quantification of immune populations from non-cancer and PDAC patients. Granulocytes are denoted as Gran, and monocytes are denoted as Mono. Biological replicates represent staining and analyses of each individual sample. Individual biological replicates were combined into box and whisker plots representing the median and interquartile range (25–75%) with whiskers representing the outlying 25%. All statistical comparisons are unpaired t-tests with a Welch’s correction.