FIG. 1.

(A) Maps of egfp constructs used for transformations. The black boxes represent the first intron from the gpd gene (55 bp). The hatched boxes 3′ and 5′ to the intron represent the coding sequence of the gpd gene. The positions of the gpd promoter (white), the mnp1 promoter (white), the egfp gene (checkered), and the mnp1 3′ UTR (horizontally striped) are indicated. (B) Restriction map of pUGGM3′, containing the gpd promoter, egfp coding region, and mnp 3′ UTR in pUB. The positions of these gene fragments with respect to the ura1 and the ampR genes are indicated and were the same in the other constructs. (C) Sequences surrounding and including the 5′ inserted intron in pUGiGM3′ and pUMGiGM3′. pUMGM3′ is used as a control. The 5′ mnp and the 5′ gpd UTR sequences are in lowercase. The intron sequences are in lowercase and are underlined. The two short gpd coding sequences are in bold uppercase, and the gfp sequences are in uppercase.