FIG. 5.

Effect of Mn supplementation on the production of extracellular MnP and intracellular GFP. Nitrogen-limited cultures of transformant T-5 were grown in the presence of 180 μm Mn or no additional Mn from a conidial inoculum as described in the text. MnP activity from duplicate cultures in the presence of 180 μM (▴) or <1 μM (▵) Mn and GFP fluorescence from duplicate cultures grown in the presence of 180 μM (■) or <1 μM (□) Mn were assayed as described in the text. Experiments were run in triplicate, and the deviations are shown.