FIG. 6.

Induction of extracellular MnP activity and intracellular GFP by Mn. Nitrogen-limited or nitrogen-sufficient, Mn-deficient cultures of transformant T-5 were grown for 5 days, after which 180 μM MnSO₄ was added to the experimental flasks. GFP fluorescence in nitrogen-limited cultures induced with Mn (■) or not induced (□) and in nitrogen-sufficient cultures induced with Mn (●) was assayed as described in the text. MnP activity in nitrogen-limited cultures induced with Mn (▴) or not induced (▵) and in nitrogen-sufficient cultures induced with Mn (○) was assayed as described in the text. Experiments were run in triplicate, and the deviations are shown.